Spatial changes of soil solution and mineral composition in the rhizosphere of Norway‐spruce seedlings colonized by Piloderma croceum

Ectomycorrhizae (ECM) or the root‐fungal association in forest ecosystems provide a unique soil microenvironment where soil properties and processes differ from the bulk soil. In this study, we would like to better understand the role of ECM systems in mineral weathering and its implications to soil formation and nutrient cycling in forest ecosystems. Specifically, we would like to document the spatial variations in the composition of soil solution and mineralogy of the rhizosphere as influenced by the ECM of Norway spruce + Piloderma croceum. Two‐month‐old seedlings of Norway spruce (control and colonized by P. croceum) were cultivated in special rhizotrons designed to allow spatial collection of soil solution. We used A and C horizons of a Dystric Cambisol collected from Höglwald forest near Munich. Micro suction cups (5 mm x 1mm) were installed in colonized and control rhizotrons, and soil solution was collected from September to November 2000. Our results show that the concentrations of NH , Ca²⁺, and Mg²⁺ in the soil solution were lower in <1.0 cm than in >3.0 cm distance from the roots of Norway spruce, due to the possible range of influence of Piloderma mycelium reaching about 2–3 cm from the surface of the mycorrhizal root. In the rhizotron with soil from the A horizon, a higher phosphorus content in Piloderma‐colonized seedlings was observed. X‐ray diffraction data indicate that chlorite and possibly mica are being transformed to 2:1‐expanding clay minerals (probably smectite) within <1.0 cm distance from roots. The spatial variations in soil solution composition and mineral transformation are likely to be due to Piloderma colonization and concentrated mycelial growth within <1.0 cm distance from the roots. This is also evident in more intricate growth of mycelia on surfaces of micaceous minerals as compared to quartz. We assume that Piloderma modifies soil solution and mineralogy through acquisition of essential elements for its own survival and/or for the uptake by plant roots. However, the presence of spontaneous infection with wildtype ECM in the control plots may have altered the influence of Piloderma and must be taken into consideration when interpreting our results.     

香稻香气特征化合物2–乙酰基–1–吡咯啉测定方法的建立

建立了一种利用顶空固相微萃取–气相色谱法(HS–SPME–GC)检测香稻香味特征化合物2–乙酰基–1–吡咯啉(2–acetyl–1–Pyrroline,2–AP)的方法。在合成2–AP标准物质的基础上,通过正交试验得到最佳萃取条件为萃取温度60℃、萃取时间45 min。在最佳萃取条件下,该检测方法的线性范围为0.5~4.0μg/g,相对标准偏差≤8.54%,回收率为96.3%~103.5%,检出限为0.045 5μg/g,定量限为0.152μg/g。利用该方法测定6种不同香稻大米2–AP的含量,结果表明,该方法能有效地检测香米的2–AP含量。     

温室自走式弥雾机远程控制系统的设计与试验

针对温室内手动施药劳动强度大、容易造成施药人员中毒等问题,利用GSM网络技术平台,设计基于手机信息发送指令的自走式弥雾机远程控制系统。该系统采用GSM模块和手机及单片机之间的双向通信实现手机对弥雾机行走速度和喷雾流量设定,运用Keil C51V9.00软件编程,对动作流程进行决策,控制弥雾机自动喷药。试验结果表明:该系统响应时间约为3s,弥雾机停止工作时,有一定的响应距离,最大为0.59m;当行走速度为4~12cm/s,喷雾流量50~300mL/min时,雾滴分布变异系数为3%~5%,喷雾均匀性较好。该远程控制系统可以满足在温室大棚内的使用要求,与传统施药方法相比,简化了植保机械的操作过程,有利于减轻劳动强度,保障人身安全。     

常见生物显微制片方法的比较研究

对常见生物材料进行切片法和非切片法制片技术的比较,结果表明:不同的生物材料、不同的组织、选择不同的制片方法,可以提高生物显微制片的质量和效率,为生物显微制片技术的研究提供参考。     

浅谈多媒体教学对数学教学的影响

多媒体技术在教学中的应用对高度抽象化的数学教学带来了很大的帮助.本文讨论了多媒体教学对数学教学的影响和运用过程中应注意的一些问题.     

微生态营养素对鱼苗池浮游生物的影响

通过在鱼苗培育过程中使用微生态营养素和传统养殖模式进行对比,分析测定养殖环境中的浮游生物种类、数量和生物量以及透明度等理化因子,研究微生态营养素在苗种培育中的使用效果。研究结果表明:微生态营养素能有效促进浮游生物的生长,施放微生态营养素的水体中鱼类喜食且易消化的浮游植物及浮游动物的种类和数量显著增加,总生物量提高了20%~58%,水体透明度降低了30%～52%,培育出来的苗种,较常规培育的个体更大、成活率更高。     

Microcracking and Chloride Penetration of Concrete under Uniaxial Compression

A non-destructive method of evaluation of specific crack area was used to characterise microcracking in concrete prisms during uniaxial compression loading and unloading. Chloride profiles were also measured after the same concretes were exposed to chloride environment. The relation among microcracking, stress-strength ratio and chloride penetration of concrete were analysed based on the experimental results. Under compressive load, the apparent chloride diffusion coefficient decreases until about one third of the ultimate load. Further increase of compressive load improves the chloride diffusion coefficient again. There is a close link between microcracking and stress-strength ratio. The index of specific crack area can be used to indicate the microcracking and study chloride transport behaviour into concrete under the influence of microcracking.     

微喷周期对葡萄叶片SPAD值和叶绿素含量与产量的影响分析

为研究极端干旱区微喷灌水技术对葡萄生理和产量的影响,通过对葡萄不同生育期叶片SPAD值、叶绿素含量和产量的测定,分析不同微喷周期条件下各处理的叶片SPAD值、叶绿素含量的变化特征和相互关系及对产量的影响。结果显示:在不同喷水周期条件下,各处理的SPAD值呈先减小后增大的变化趋势,日变化值为38.31~43.8。其中,每天喷水1h处理的SPAD值日平均值最高,为42.0,之后是对照处理和每隔1d喷水2h处理,分别为41.2和40.3,每隔2d喷水3h处理的SPAD值最低,为39.3。葡萄SPAD值与叶绿素a、叶绿素b以及总叶绿素含量间相关关系显著,回归方程分别为Ca=0.1028VSPAD-2.2266、Cb=0.0796VSPAD-2.3749、Ct=0.1824VSPAD-4.4602。在葡萄品质指标和产量上,采用微喷可以提高VC含量、多酚含量和单宁含量等多项品质指标,并使葡萄平均增产5.4%,表明合理的微喷周期处理有利于提高葡萄叶片叶绿素含量,并使产量增加,而叶绿素仅是葡萄生理生长的指标之一,如光合、叶绿素荧光等指标对葡萄生理的影响仍需作更深入的研究,以期能更进一步揭示微喷条件下的葡萄增产作用机理。     

Nutrient release pattern of organic and inorganic resources used in cassava production (Manihot esculenta Crantz)

The major constraint in the promotion of organic farming, a safe and sustainable alternative, is lack of availability of quality organic resources in sufficient quantities. The quality of resources can be ascertained by monitoring its nutrient release pattern, which has not yet been attempted. A controlled condition pot experiment was conducted at ICAR-Central Tuber Crops Research Institute, Thiruvananthapuram, India, in completely randomized design to study the nutrient dynamics of various organic and inorganic resources, commonly used in cassava production, at monthly intervals up to six months. The release of almost all nutrients and activity of soil enzymes were higher at the middle (3 or 4?months) of the incubation period. The pH showed an increasing trend and electrical conductivity, organic C and Fe content declined from initial. Averaging over stages, organic practice favored the activity of soil enzymes and release of almost all nutrients over conventional system significantly, except N.     

Physical carbon‐sequestration mechanisms under special consideration of soil wettability

The protective impact of aggregation on microbial degradation through separation has been described frequently, especially for biotically formed aggregates. However, to date little information exists on the effects of organic‐matter (OM) quantity and OM quality on physical protection, i.e., reduced degradability by microorganisms caused by physical factors. In the present paper, we hypothesize that soil wettability, which is significantly influenced by OM, may act as a key factor for OM stabilization as it controls the microbial accessibility for water, nutrients, and oxygen in three‐phase systems like soil. Based on this hypothesis, the first objective is to evaluate new findings on the organization of organo‐mineral complexes at the nanoscale as one of the processes creating water‐repellent coatings on mineral surfaces. The second objective is to quantify the degree of alteration of coated surfaces with regard to water repellence. We introduce a recently developed trial that combines FTIR spectra with contact‐angle data as the link between chemical composition of OM and the physical wetting behavior of soil particles. In addition to characterizing the wetting properties of OM coatings, we discuss the implications of water‐repellent surfaces for different physical protection mechanisms of OM. For typical minerals, the OM loading on mineral surfaces is patchy, whereas OM forms nanoscaled micro‐aggregates together with metal oxides and hydroxides and with layered clay minerals. Such small aggregates may efficiently stabilize OM against microbial decomposition. However, despite the patchy structure of OM coating, we observed a relation between the chemical composition of OM and wettability. A higher hydrophobicity of the OM appears to stabilize the organic C in soil, either caused by a specific reduced biodegradability of OM or indirectly caused by increased aggregate stability. In partly saturated nonaggregated soil, the specific distribution of the pore water appears to further affect the mineralization of OM as a function of wettability. We conclude that the wettability of OM, quantified by the contact angle, links the chemical structure of OM with a bundle of physical soil properties and that reduced wettability results in the stabilization of OM in soils.